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Higel-Agarose clear™

  • Grade: BIOTECHNOLOGY GRADE

Overview

  • All-Purpose, High-purity Agarose
  • Routine nucleic acid analytical/preparative applications.
  • Standard melting/gelling agarose
  • Blotting techniques
  • Nuclease & Protease-free
  • Clear gel background

SPECIFICATIONS

  • Form: White powder.
  • EEO (-mr): 0.09 - 0.13
  • Gel Point (1.5%): 36 ± 1.5 degrees ℃
  • melt Point (1.5%): 88 ± 1.5 degrees ℃
  • Gel Strength (1%): ≥ 1200 g/㎠
  • Gel Strength (1.5%): ≥ 2500 g/㎠
  • Moisture: ≤ 7-8 %
  • Turbidity (1.5%): ≤ 4 NTU
  • Sulfate (SO4): ≤ 0.14%
  • DNase: None detected
  • RNase: None detected
  • Gel Background: Very clear

Usage

Recommended Higel-Agarose clear™ concentrations for optimal resolution
GEL (%) OPTIMAL SEPARATION RANGE (bp) RECOMMENDED BUFFER
0.8 500 – 22,000 TAE
1.0 400 – 10,000 TAE/TBE
1.2 300 – 5,000 TAE/TBE
2.0 200 – 3,000 TBE
PRODUCT DESCRIPTION Catalog NO. SIZE
Higel-Agarose clear™ HB0100100 100g
  HB0100500 500g

Dissolving Agarose clear™

Method 1: Microwave

  1. Determine the amount of agarose solution needed to cast your gel.Note: Remember to take the thickness of the gel into account, as it affects both well volume and power requirements.
  2. Add room temperature buffer (TAE or TBE) into a flask that can hold 2–4 times the volume of your agarose solution. Place a magnetic stir bar into the flask.
  3. Put the flask on a magnetic stirrer and slowly sprinkle the required amount of agarose powder into the flask as the solution mixes, to prevent the formation of agarose clumps.
  4. Remove the stir bar.
  5. Weigh the flask and solution before heating.
  6. Cover the mouth of the flask with plastic wrap, and pierce the wrap with a small hole for ventilation.
  7. Place the flask in the microwave oven and heat the solution until bubbles appear.
  8. Remove the flask carefully, and swirl gently to resuspend any agarose particles. Exercise caution – microwaved solution may become superheated and foam over when agitated.
  9. Reheat the solution until the solution comes to a boil, and all the agarose particles are dissolved.
  10. Remove the flask carefully and swirl gently to mix the solution.
  11. Place the flask on a scale, and bring it back to its initial weight (from Step 5) with warm distilled water.
  12. Mix gently and cool to 50–60°C (at room temperature for at least 20 minutes) before pouring the solution into the casting tray.

Method 2: Boiling water bath

  1. Determine the amount of agarose solution needed to cast your gel. Note: Remember to take the thickness of the gel into account, as it affects both well volume and power requirements.
  2. Add room temperature buffer (TAE or TBE) into a flask that can hold 2–4 times the volume of your agarose solution. Place a magnetic stir bar into the flask.
  3. Put the flask on a magnetic stirrer and slowly sprinkle the required amount of agarose powder into the flask as the solution mixes, to prevent the formation of agarose clumps.
  4. Weigh the flask and solution before heating.
  5. Cover the mouth of the flask with plastic wrap, and pierce the wrap with a small hole for ventilation.
  6. Bring the solution to a boil while stirring, and allow it to boil gently for approximately 10 minutes or until the agarose is completely dissolved.
  7. Place the flask on a scale, and bring it back to its initial weight (from Step 4) with warm distilled water.
  8. Mix gently and cool to 50-60°C (at room temperature for at least 20 minutes) before pouring the solution into the casting tray.

Dye Mobility

Refer to the following table for the migration of Bromophenol Blue and Xylene Cyanol tracking dyes in relation to DNA:

Agarose(w/v%) Bromophenol Blue Xylene Cyanol
TAE TBE TAE TBE
0.3 2,900 2,850 24,800 19,400
0.5 1,650 1,350 11,000 12,000
0.75 1,000 720 10,200 9,200
1 500 400 6,100 4,100
1.25 370 260 3,560 2,500
1.5 300 200 2,800 1,800
1.75 200 110 1,800 1,100
2 150 70 1,300 850

※ Higel-Agarose clear™은 (주)E&S 사의 등록상표입니다.